destaining buffer (Bio-Rad)
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destaining buffer
Destaining Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 158 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/destaining+buffer/Coomassie+Brilliant+Blue+R-250+Destaining+Solution/10__22424_slash_jdsb__2023__41__3__138-69-15-23
Average 95 stars, based on 158 article reviews
Destaining Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 158 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/destaining+buffer/Coomassie+Brilliant+Blue+R-250+Destaining+Solution/10__22424_slash_jdsb__2023__41__3__138-69-15-23
Average 95 stars, based on 158 article reviews
destaining buffer - by Bioz Stars,
2026-11
95/100 stars
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Incubation:Article Title: Effect of aluminium on migration of oestrogen unresponsive MDA-MB-231 human breast cancer cells in culture. Article Snippet: Aluminium (Al) has been measured in human breast tissue, and may be a contributory factor in breast cancer development.. At the 10 th Keele meeting, we reported that longterm exposure to Al could increase migratory properties of oestrogen-responsive MCF-7 human breast cancer cells suggesting a role for Al in the metastatic process.. We now report that long-term exposure (20-25 weeks) to Al chloride or Al chlorohydrate at 10 -4 M or 10 -5 M concentrations can also increase the migration of oestrogen unresponsive MDA-MB-231 human breast cancer cells as measured using time-lapse microscopy and xCELLigence technology. Article Title: Maternal hypoxia alters matrix metalloproteinase expression patterns and causes cardiac remodeling in fetal and neonatal rats Article Snippet: .. The gel was renatured by renaturation buffer (Bio-Rad) for 1 h and then incubated with a development buffer (Bio-Rad) at 37°C for 48 h. The gel was stained with 0.5% Coomassie blue R-250 (Bio-Rad) for 1 h and then destained with Article Title: The p75(NTR) metastasis suppressor inhibits urokinase plasminogen activator, matrix metalloproteinase-2 and matrix metalloproteinase-9 in PC-3 prostate cancer cells. Article Snippet: The p75 neurotrophin receptor (p75) has been characterized as a metastasis and tumor suppressor in prostate cancer.. In order to investigate the mechanism(s) by which the p75 functions as a metastasis suppressor in prostate cancer cells, we characterized the ectopic expression of p75 on the urokinase plasminogen activator (uPA) and the type IV collagen matrix metalloproteinases (MMP-2 and MMP-9) in PC-3 human prostate cancer cells.. Rank-order expression of p75 greatly reduced protein levels and enzymatic activities of uPA, MMP-2, and MMP-9 as shown by immunoblot and zymography analyses. Staining:Article Title: Effect of aluminium on migration of oestrogen unresponsive MDA-MB-231 human breast cancer cells in culture. Article Snippet: Aluminium (Al) has been measured in human breast tissue, and may be a contributory factor in breast cancer development.. At the 10 th Keele meeting, we reported that longterm exposure to Al could increase migratory properties of oestrogen-responsive MCF-7 human breast cancer cells suggesting a role for Al in the metastatic process.. We now report that long-term exposure (20-25 weeks) to Al chloride or Al chlorohydrate at 10 -4 M or 10 -5 M concentrations can also increase the migration of oestrogen unresponsive MDA-MB-231 human breast cancer cells as measured using time-lapse microscopy and xCELLigence technology. Article Title: Single-Administration Long-Acting Microarray Patch with Ultrahigh Loading Capacity and Multiple Releases of Thermally Stable Antibodies. Article Snippet: Twenty microliters of each sample was mixed with a Laemmli sample buffer (Bio-Rad Laboratories, Inc., USA) at 1:1 v/v ratio and incubated at 90 °C for 10 min. Then, 20 μL of each sample was loaded per well (8−16% Mini-PROTEAN TGX stain-free protein gels, Bio-Rad Laboratories, Inc., USA), and run for 20 min at 220 V (PowerPac Basic Power Supply, Bio-Rad Laboratories, Inc., USA). .. The gels were Coomassie Blue stained-overnight and then destained using a Article Title: Maternal hypoxia alters matrix metalloproteinase expression patterns and causes cardiac remodeling in fetal and neonatal rats Article Snippet: .. The gel was renatured by renaturation buffer (Bio-Rad) for 1 h and then incubated with a development buffer (Bio-Rad) at 37°C for 48 h. The gel was stained with 0.5% Coomassie blue R-250 (Bio-Rad) for 1 h and then destained with Article Title: Effect of Lactobacillus acidophilus Bauer and Bifidobacterium animalis ssp. lactis BB12 on proteolytic changes in dry-cured loins Article Snippet: Subsequently, electrophoresis was conducted at a constant voltage of 40 V for ~6 h. After electrophoresis, the gel was placed in the staining buffer (Coomassie Brilliant Blue R-250 Staining Solution manufactured by Bio-Rad). .. After 1 h of staining, the gel was placed in the Article Title: Single-Administration Long-Acting Microarray Patch with Ultrahigh Loading Capacity and Multiple Releases of Thermally Stable Antibodies Article Snippet: Twenty microliters of each sample was mixed with a Laemmli sample buffer (Bio-Rad Laboratories, Inc., USA) at 1:1 v/v ratio and incubated at 90 °C for 10 min. Then, 20 μL of each sample was loaded per well (8–16% Mini-PROTEAN TGX stain-free protein gels, Bio-Rad Laboratories, Inc., USA), and run for 20 min at 220 V (PowerPac Basic Power Supply, Bio-Rad Laboratories, Inc., USA). .. The gels were Coomassie Blue stained-overnight and then destained using a Article Title: The p75(NTR) metastasis suppressor inhibits urokinase plasminogen activator, matrix metalloproteinase-2 and matrix metalloproteinase-9 in PC-3 prostate cancer cells. Article Snippet: The p75 neurotrophin receptor (p75) has been characterized as a metastasis and tumor suppressor in prostate cancer.. In order to investigate the mechanism(s) by which the p75 functions as a metastasis suppressor in prostate cancer cells, we characterized the ectopic expression of p75 on the urokinase plasminogen activator (uPA) and the type IV collagen matrix metalloproteinases (MMP-2 and MMP-9) in PC-3 human prostate cancer cells.. Rank-order expression of p75 greatly reduced protein levels and enzymatic activities of uPA, MMP-2, and MMP-9 as shown by immunoblot and zymography analyses. Article Title: Growth Characteristics of Bacteriocin-Producing Lactococcus lactis subsp. hordniae JNU533 in a Glucose-Containing Skim Milk Medium Article Snippet: .. The gel was stained with Coomassie Brilliant Blue R-250 (Bio-Rad Laboratories, USA) and washed with Gentle:Article Title: The p75(NTR) metastasis suppressor inhibits urokinase plasminogen activator, matrix metalloproteinase-2 and matrix metalloproteinase-9 in PC-3 prostate cancer cells. Article Snippet: The p75 neurotrophin receptor (p75) has been characterized as a metastasis and tumor suppressor in prostate cancer.. In order to investigate the mechanism(s) by which the p75 functions as a metastasis suppressor in prostate cancer cells, we characterized the ectopic expression of p75 on the urokinase plasminogen activator (uPA) and the type IV collagen matrix metalloproteinases (MMP-2 and MMP-9) in PC-3 human prostate cancer cells.. Rank-order expression of p75 greatly reduced protein levels and enzymatic activities of uPA, MMP-2, and MMP-9 as shown by immunoblot and zymography analyses. Activity Assay:Article Title: The p75(NTR) metastasis suppressor inhibits urokinase plasminogen activator, matrix metalloproteinase-2 and matrix metalloproteinase-9 in PC-3 prostate cancer cells. Article Snippet: The p75 neurotrophin receptor (p75) has been characterized as a metastasis and tumor suppressor in prostate cancer.. In order to investigate the mechanism(s) by which the p75 functions as a metastasis suppressor in prostate cancer cells, we characterized the ectopic expression of p75 on the urokinase plasminogen activator (uPA) and the type IV collagen matrix metalloproteinases (MMP-2 and MMP-9) in PC-3 human prostate cancer cells.. Rank-order expression of p75 greatly reduced protein levels and enzymatic activities of uPA, MMP-2, and MMP-9 as shown by immunoblot and zymography analyses. |